26430
Sigma
Chlortetracycline hydrochloride
BioChemika, from Streptomyces aureofaciens, for fluorescence, ≥97.0% (AT)
| Synonym: | 7-Chlorotetracycline hydrochloride |
| CAS Number: | 64-72-2 |
| Linear Formula: | C22H23ClN2O8 · HCl |
| Molecular Weight: | 515.34 |
| Beilstein Registry Number: | 3858364 |
| EC Number: | 200-591-7 |
| MDL number: | MFCD00082440 |
| PubChem Substance ID: | 24856004 |
Description
| Application | Cell-permeable fluorescent probe for Ca2+ for measuring calcium flux in endoplasmic reticulum and other intracellular stores in situ. |
| Biochem/physiol Actions | Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-positive. Less active against Gram-negative bacteria than tetracycline. Mode of Resistance: Loss of cell wall permeability. |
| Quality | Recommended for fluorescent studies of calcium signaling in cells or tissues. |
Properties
| biological source | from Streptomyces aureofaciens |
| grade | for fluorescence |
| product line | BioChemika |
| assay | ≥97.0% (AT) |
| total impurities | ≤3% water |
| fluorescence | λex 392 nm; λem 536 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+ |
| mp | 210-215 °C (dec.)(lit.) |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| WGK Germany | 2 |
| RTECS | QI7800000 |
References
| reference | Cerella, C., et al., Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline. Ann. N.Y. Acad. Sci. 1099, 490-493, (2007) Abstract |
| Agwuh, K.N., and MacGowan, A., Pharmacokinetics and pharmacodynamics of the tetracyclines including glycylcyclines. J. Antimicrob. Chemother. 58, 256-265, (2006) Abstract | |
| Gillan, L., et al., Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 63, 445-457, (2005) Abstract | |
| Oliver, A.E., et al., Effects of temperature on calcium-sensitive fluorescent probes. Biophys. J. 78, 2116-2126, (2000) Abstract | |
| G. Schwartzman et al. Analytical Profiles of Drug Substances New York , (1979) 8, 101 | |
| Merck | Merck 13,2211 |
| Beilstein | Beil. 14,IV,2631 |
| Corp MSDS 1 (1), 871:B / RegBook 1 (2), 2097:N / Sax 6, 786 / Sigma FT-IR 1 (1), 637:A / Structure Index 1, 332:D:1 |
